Probe-Based Multiplex Real-Time PCR as a Diagnostic Tool to Distinguish Distinct Fungal Symbionts Associated With Euwallacea kuroshio and Euwallacea whitfordiodendrus in California.
Identifieur interne : 000182 ( Main/Exploration ); précédent : 000181; suivant : 000183Probe-Based Multiplex Real-Time PCR as a Diagnostic Tool to Distinguish Distinct Fungal Symbionts Associated With Euwallacea kuroshio and Euwallacea whitfordiodendrus in California.
Auteurs : Joseph D. Carrillo [États-Unis] ; Joey S. Mayorquin [États-Unis] ; Jason E. Stajich [États-Unis] ; Akif Eskalen [États-Unis]Source :
- Plant disease [ 0191-2917 ] ; 2020.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Ascomycota, Fusarium.
- microbiologie : Charançons.
- Animaux, Ascomycota, Californie, Espèce introduite, Femelle, Fusarium, Limite de détection, Réaction de polymérisation en chaine en temps réel.
English descriptors
- KwdEn :
- MESH :
- geographic : California.
- classification : Ascomycota, Fusarium.
- genetics : Ascomycota, Fusarium.
- microbiology : Weevils.
- Animals, Female, Introduced Species, Limit of Detection, Real-Time Polymerase Chain Reaction.
Abstract
California has been invaded by two distinct Euwallacea spp. that vector unique plant pathogenic symbiotic fungi on multiple hosts and cause Fusarium dieback. The objective of this study was to develop multiplex real-time quantitative PCR assays using hydrolysis probes targeting the β-tubulin gene to detect, distinguish, and quantify fungi associated with the polyphagous shot hole borer (PSHB; Euwallacea whitfordiodendrus, Fusarium euwallaceae, Graphium euwallaceae, and Paracremonium pembeum) as well as the Kuroshio shot hole borer (KSHB; Euwallacea kuroshio, Fusarium kuroshium, and Graphium kuroshium) from various sample types. Absolute quantification reaction efficiencies ranged from 88.2 to 104.3%, with a coefficient of determination >0.992 and a limit of detection of 100 copies µl-1 for all targets across both assays. Qualitative detection using the real-time assays on artificially inoculated avocado shoot extracts showed more sensitivity compared with conventional fungal isolation from wood. All symbiotic fungi, except P. pembeum, from PSHB and KSHB female heads were detectable and quantified. Field samples from symptomatic Platanus racemosa, Populus spp., and Salix spp. across 17 of 26 city parks were positively identified as PSHB and KSHB through detection of their symbiotic fungi, and both were found occurring together on five trees from three different park locations. The molecular assays presented here can be utilized to accurately identify fungi associated with these invasive pests in California.
DOI: 10.1094/PDIS-01-19-0201-RE
PubMed: 31647694
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Probe-Based Multiplex Real-Time PCR as a Diagnostic Tool to Distinguish Distinct Fungal Symbionts Associated With <i>Euwallacea kuroshio</i> and <i>Euwallacea whitfordiodendrus</i> in California.</title><author><name sortKey="Carrillo, Joseph D" sort="Carrillo, Joseph D" uniqKey="Carrillo J" first="Joseph D" last="Carrillo">Joseph D. Carrillo</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Microbiology and Plant Pathology, University of California, Riverside</wicri:cityArea></affiliation></author><author><name sortKey="Mayorquin, Joey S" sort="Mayorquin, Joey S" uniqKey="Mayorquin J" first="Joey S" last="Mayorquin">Joey S. Mayorquin</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Microbiology and Plant Pathology, University of California, Riverside</wicri:cityArea></affiliation></author><author><name sortKey="Stajich, Jason E" sort="Stajich, Jason E" uniqKey="Stajich J" first="Jason E" last="Stajich">Jason E. Stajich</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Microbiology and Plant Pathology, University of California, Riverside</wicri:cityArea></affiliation></author><author><name sortKey="Eskalen, Akif" sort="Eskalen, Akif" uniqKey="Eskalen A" first="Akif" last="Eskalen">Akif Eskalen</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Pathology, University of California, Davis, CA 95616.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Plant Pathology, University of California, Davis</wicri:cityArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:31647694</idno><idno type="pmid">31647694</idno><idno type="doi">10.1094/PDIS-01-19-0201-RE</idno><idno type="wicri:Area/Main/Corpus">000649</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000649</idno><idno type="wicri:Area/Main/Curation">000649</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000649</idno><idno type="wicri:Area/Main/Exploration">000649</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Probe-Based Multiplex Real-Time PCR as a Diagnostic Tool to Distinguish Distinct Fungal Symbionts Associated With <i>Euwallacea kuroshio</i> and <i>Euwallacea whitfordiodendrus</i> in California.</title><author><name sortKey="Carrillo, Joseph D" sort="Carrillo, Joseph D" uniqKey="Carrillo J" first="Joseph D" last="Carrillo">Joseph D. Carrillo</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Microbiology and Plant Pathology, University of California, Riverside</wicri:cityArea></affiliation></author><author><name sortKey="Mayorquin, Joey S" sort="Mayorquin, Joey S" uniqKey="Mayorquin J" first="Joey S" last="Mayorquin">Joey S. Mayorquin</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Microbiology and Plant Pathology, University of California, Riverside</wicri:cityArea></affiliation></author><author><name sortKey="Stajich, Jason E" sort="Stajich, Jason E" uniqKey="Stajich J" first="Jason E" last="Stajich">Jason E. Stajich</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Microbiology and Plant Pathology, University of California, Riverside</wicri:cityArea></affiliation></author><author><name sortKey="Eskalen, Akif" sort="Eskalen, Akif" uniqKey="Eskalen A" first="Akif" last="Eskalen">Akif Eskalen</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Pathology, University of California, Davis, CA 95616.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Plant Pathology, University of California, Davis</wicri:cityArea></affiliation></author></analytic><series><title level="j">Plant disease</title><idno type="ISSN">0191-2917</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Ascomycota (classification)</term><term>Ascomycota (genetics)</term><term>California (MeSH)</term><term>Female (MeSH)</term><term>Fusarium (classification)</term><term>Fusarium (genetics)</term><term>Introduced Species (MeSH)</term><term>Limit of Detection (MeSH)</term><term>Real-Time Polymerase Chain Reaction (MeSH)</term><term>Weevils (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Ascomycota (classification)</term><term>Ascomycota (génétique)</term><term>Californie (MeSH)</term><term>Charançons (microbiologie)</term><term>Espèce introduite (MeSH)</term><term>Femelle (MeSH)</term><term>Fusarium (classification)</term><term>Fusarium (génétique)</term><term>Limite de détection (MeSH)</term><term>Réaction de polymérisation en chaine en temps réel (MeSH)</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>California</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Ascomycota</term><term>Fusarium</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Ascomycota</term><term>Fusarium</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Ascomycota</term><term>Fusarium</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Charançons</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Weevils</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Introduced Species</term><term>Limit of Detection</term><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Ascomycota</term><term>Californie</term><term>Espèce introduite</term><term>Femelle</term><term>Fusarium</term><term>Limite de détection</term><term>Réaction de polymérisation en chaine en temps réel</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">California has been invaded by two distinct <i>Euwallacea</i> spp. that vector unique plant pathogenic symbiotic fungi on multiple hosts and cause Fusarium dieback. The objective of this study was to develop multiplex real-time quantitative PCR assays using hydrolysis probes targeting the β-tubulin gene to detect, distinguish, and quantify fungi associated with the polyphagous shot hole borer (PSHB; <i>Euwallacea whitfordiodendrus</i>, <i>Fusarium euwallaceae</i>, <i>Graphium euwallaceae</i>, and <i>Paracremonium pembeum</i>) as well as the Kuroshio shot hole borer (KSHB; <i>Euwallacea kuroshio</i>, <i>Fusarium kuroshium</i>, and <i>Graphium kuroshium</i>) from various sample types. Absolute quantification reaction efficiencies ranged from 88.2 to 104.3%, with a coefficient of determination >0.992 and a limit of detection of 100 copies µl<sup>-1</sup> for all targets across both assays. Qualitative detection using the real-time assays on artificially inoculated avocado shoot extracts showed more sensitivity compared with conventional fungal isolation from wood. All symbiotic fungi, except <i>P. pembeum</i>, from PSHB and KSHB female heads were detectable and quantified. Field samples from symptomatic <i>Platanus racemosa</i>, <i>Populus</i> spp., and <i>Salix</i> spp. across 17 of 26 city parks were positively identified as PSHB and KSHB through detection of their symbiotic fungi, and both were found occurring together on five trees from three different park locations. The molecular assays presented here can be utilized to accurately identify fungi associated with these invasive pests in California.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">31647694</PMID><DateCompleted><Year>2020</Year><Month>01</Month><Day>28</Day></DateCompleted><DateRevised><Year>2020</Year><Month>01</Month><Day>28</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0191-2917</ISSN><JournalIssue CitedMedium="Print"><Volume>104</Volume><Issue>1</Issue><PubDate><Year>2020</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Plant disease</Title><ISOAbbreviation>Plant Dis</ISOAbbreviation></Journal><ArticleTitle>Probe-Based Multiplex Real-Time PCR as a Diagnostic Tool to Distinguish Distinct Fungal Symbionts Associated With <i>Euwallacea kuroshio</i> and <i>Euwallacea whitfordiodendrus</i> in California.</ArticleTitle><Pagination><MedlinePgn>227-238</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PDIS-01-19-0201-RE</ELocationID><Abstract><AbstractText>California has been invaded by two distinct <i>Euwallacea</i> spp. that vector unique plant pathogenic symbiotic fungi on multiple hosts and cause Fusarium dieback. The objective of this study was to develop multiplex real-time quantitative PCR assays using hydrolysis probes targeting the β-tubulin gene to detect, distinguish, and quantify fungi associated with the polyphagous shot hole borer (PSHB; <i>Euwallacea whitfordiodendrus</i>, <i>Fusarium euwallaceae</i>, <i>Graphium euwallaceae</i>, and <i>Paracremonium pembeum</i>) as well as the Kuroshio shot hole borer (KSHB; <i>Euwallacea kuroshio</i>, <i>Fusarium kuroshium</i>, and <i>Graphium kuroshium</i>) from various sample types. Absolute quantification reaction efficiencies ranged from 88.2 to 104.3%, with a coefficient of determination >0.992 and a limit of detection of 100 copies µl<sup>-1</sup> for all targets across both assays. Qualitative detection using the real-time assays on artificially inoculated avocado shoot extracts showed more sensitivity compared with conventional fungal isolation from wood. All symbiotic fungi, except <i>P. pembeum</i>, from PSHB and KSHB female heads were detectable and quantified. Field samples from symptomatic <i>Platanus racemosa</i>, <i>Populus</i> spp., and <i>Salix</i> spp. across 17 of 26 city parks were positively identified as PSHB and KSHB through detection of their symbiotic fungi, and both were found occurring together on five trees from three different park locations. The molecular assays presented here can be utilized to accurately identify fungi associated with these invasive pests in California.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Carrillo</LastName><ForeName>Joseph D</ForeName><Initials>JD</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-1790-6776</Identifier><AffiliationInfo><Affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mayorquin</LastName><ForeName>Joey S</ForeName><Initials>JS</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stajich</LastName><ForeName>Jason E</ForeName><Initials>JE</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Plant Pathology, University of California, Riverside, CA 92521.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Eskalen</LastName><ForeName>Akif</ForeName><Initials>A</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-8829-7413</Identifier><AffiliationInfo><Affiliation>Department of Plant Pathology, University of California, Davis, CA 95616.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>10</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Dis</MedlineTA><NlmUniqueID>9882809</NlmUniqueID><ISSNLinking>0191-2917</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001203" MajorTopicYN="Y">Ascomycota</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002140" MajorTopicYN="N" Type="Geographic">California</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005670" MajorTopicYN="Y">Fusarium</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058865" MajorTopicYN="N">Introduced Species</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D057230" MajorTopicYN="N">Limit of Detection</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="Y">Real-Time Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D048889" MajorTopicYN="Y">Weevils</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">ambrosia beetle</Keyword><Keyword MajorTopicYN="N">pathogen detection</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>10</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>1</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>10</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31647694</ArticleId><ArticleId IdType="doi">10.1094/PDIS-01-19-0201-RE</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Carrillo, Joseph D" sort="Carrillo, Joseph D" uniqKey="Carrillo J" first="Joseph D" last="Carrillo">Joseph D. Carrillo</name></region><name sortKey="Eskalen, Akif" sort="Eskalen, Akif" uniqKey="Eskalen A" first="Akif" last="Eskalen">Akif Eskalen</name><name sortKey="Mayorquin, Joey S" sort="Mayorquin, Joey S" uniqKey="Mayorquin J" first="Joey S" last="Mayorquin">Joey S. Mayorquin</name><name sortKey="Stajich, Jason E" sort="Stajich, Jason E" uniqKey="Stajich J" first="Jason E" last="Stajich">Jason E. Stajich</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000182 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000182 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:31647694
|texte= Probe-Based Multiplex Real-Time PCR as a Diagnostic Tool to Distinguish Distinct Fungal Symbionts Associated With Euwallacea kuroshio and Euwallacea whitfordiodendrus in California.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:31647694" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |